Product Name: Tris(hydroxymethyl)aminomethane
CAS: 77-86-1
MF: C4H11NO3
MW: 121.14
EINECS: 201-064-4
Melting point: 167-172 °C(lit.)
Boiling point: 219-220 °C10 mm Hg(lit.)
Bulk density: 840kg/m3
Density: 1,353 g/cm3
Vapor pressure: 0.0267 hPa (20 °C)
Refractive index: 1.4170 (estimate)
Fp: 219-220°C/10mm
Storage temp.: 20-25°C
Tris(hydroxymethyl)aminomethane, commonly known as Tris, is a widely used compound, especially in the fields of biochemistry and molecular biology.
1. Tris CAS 77-86-1 is primarily used as a buffer in biological and biochemical research. It helps maintain a stable pH in solution, Tris is essential for many biochemical reactions and processes.
2. Tris is often used in gel electrophoresis, especially when preparing electrophoresis buffers for DNA and RNA separations. It helps maintain pH during electrophoresis.
3. In cell culture medium, Tris CAS 77-86-1 can be used to help maintain the pH value of the culture medium, providing a stable environment for cell growth and experiments.
4. Tris is commonly used in various protein purification techniques, including chromatography, where maintaining a specific pH is important for protein stability and activity.
5. Tris CAS 77-86-1 is used in various molecular biology techniques including PCR (polymerase chain reaction) and DNA extraction protocols, where a stable pH is essential for enzyme activity.
6. Tris is widely used in research fields, including those involving enzyme kinetics, protein interactions, and nucleic acid studies.
Packed in 25 kg per drum or based on customers' requirements.
Uses of Tris(hydroxymethyl)aminomethane
Tris(hydroxymethyl)aminomethane, commonly referred to as Tris, is widely used in various fields, especially in biochemistry and molecular biology.
1. Tris CAS 77-86-1 is primarily used as a buffer in biological and biochemical research. It helps maintain a stable pH in solution, Tris is essential for many biochemical reactions and processes.
2. Tris is commonly used in gel electrophoresis, especially in the preparation of running buffers for DNA and RNA separations.
3. Tris CAS 77-86-1 used to maintain pH stability in cell culture medium, which is important for cell growth and function.
4. Tris is often included in buffers used for protein purification and analysis, such as in chromatography techniques.
5. Tris CAS 77-86-1 is used in various molecular biology techniques including PCR (polymerase chain reaction) and DNA extraction protocols.
6. Store Tris in airtight containers made of suitable materials, such as glass or high-density polyethylene (HDPE). Ensure the container is clearly labeled with the chemical name and any relevant hazard information.
7. Store Tris CAS 77-86-1 in a cool, dry, well-ventilated place away from direct sunlight, heat and moisture.
8. Although Tris is relatively stable, it is best stored at room temperature (usually 15°C to 25°C or 59°F to 77°F). Avoid exposure to extreme temperatures.
9. Tris CAS 77-86-1 is generally considered harmless, but keep it separate from incompatible substances. Also, keep it away from food and drinks.
10. Regularly inspect storage areas and containers for signs of leaks, damage or deterioration.